ACB (auto contrast/brightness) of M-D acq

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ACB (auto contrast/brightness) of M-D acq

Tsengming Chou
ACB (auto contrast/brightness) of M-D acq I kind of remember a discuss (while ago) regarding to the ACB (auto contrast brightness) in the image stack of acquired M-D data.  It seems like the ACB is turned “ON” as the default.  Is there a way to turn off the ACB while collecting M-D data?

Thanks in advance,

Alex
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Dr. Tsengming (Alex) Chou
Staff Scientist
Laboratory for Multiscale Imaging (LMSI)
Stevens Institute of Technology

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Re: ACB (auto contrast/brightness) of M-D acq

Nico Stuurman-4
Hi Alex,

I kind of remember a discuss (while ago) regarding to the ACB (auto contrast brightness) in the image stack of acquired M-D data.  It seems like the ACB is turned “ON” as the default.  Is there a way to turn off the ACB while collecting M-D data?

The "ACB" feature has been changed in one of the latest release, and it now determined the brightest and darkest pixel of the whole image stack of the first time point (rather than just the first image) for each channel, which should make it much more useful for most people.  There is currently no way of shutting auto-contrasting off completely.  However, you can always manually adjust brightness/contrast in a stack, or you could use a very small script to do so.

Best,

Nico


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