> Hi Lukas,
>
> I am afraid that I can not help much at this point in time, but we ran
> into similar issues with our dual camera setup (2 iXons), and we are
> still using the "old" Andor adaptor code on our dual camera
> microscope. The Andor engineer at the time told me that he could
> reproduce the issues I was seeing (will have to go back to see if
> those were the same you were seeing) with VS2010, but that they went
> away with the newer VS that he was using. Although that did not make
> a lot of sense to me at the time, I very much do want to see MM to
> migrate soon to new compilers, to at least make sure whether or not
> that is an issue.
>
> It would be great if you could contact Andor technical support about
> these issues, and see if they can raise it to a point that their
> software developers get involved. At that point, please do get me in
> the loop as well. Andor maintains the device adaptor code, and the
> April 2019 update was quite drastic, but certainly left a number of
> issues behind. I am afraid that they are in the best position to fix
> this (but I'll be happy to advice).
>
> Best,
>
>
> Nico
>
>
>> Hi Pedro,
>>
>> I am using the 64bit version.
>> ImageJ.cfg is set with -Xmx6000m
>> The Sequence Buffer Size is 512MB which can hold 2048 images.
>> The computer has 32Gb of RAM.
>>
>> Single Andor camera:
>> With the old adapter the average return time of
>> mmc.popNextTaggedImage(); is 1ms (2x binning --> 256x256 pixel, 16bit)
>> With the new adapter (past 14.April19) the average return time of
>> mmc.popNextTaggedImage(); is 18ms (exactly the same config file, just
>> changed mmgr_dal_Andor.dll)
>>
>> With the Multi Camera adapter:
>> old adapter: 2ms
>> new adapter: 36ms --> not sufficient for 100fps (2x 50fps)
>>
>> I am also curious why the Multi Camera adapter doubles the time to
>> return a single image.
>>
>> With the "new" adapter the Sequence Buffer fills up quite fast
>> (observed with Sequence Buffer Monitor).
>> With the "old" adapter the Sequence Buffer Monitor shows rarely 1-2
>> images.
>> I can't just increase the buffer size, it would exceed due to the
>> long recording time.
>> Also: the System is tracking on the camera stream, therefore a large
>> amount of images in a huge buffer causes a fatal delay for the tracker.
>>
>> Further Interesting:
>> With "Trigger: Software", on the "new" adapter the cameras are
>> slowing down a lot and therefore the buffer is kept below 100 images..
>> But with external Trigger the buffer overflow occurs quite quick (but
>> the cameras stay at pace).
>> The readout time of the cameras is indicated with about 16ms.
>>
>> The script i used to investigate in the timings:
>>
>> mm.scripter().resetInterpreter(); //Reset Interpreter to get rid of
>> old stuff
>> mm.scripter().clearMessageWindow(); //Clear Script output window
>> /**
>> * A burst aquisition test script
>> */
>> import org.micromanager.interal.utils.JavaUtils;
>> //import org.micromanager.data.Datastore;
>>
>> // User defined variables:
>> int nrFrames = 1000;
>> int loopSleepTime = 3;
>>
>> // defining variables
>> long taggedTime = 0;
>> long endSequenceTime = 0;
>> int loopCount = 0;
>>
>> // stop running acquisitions
>> if (mmc.isSequenceRunning()) {
>> mmc.stopSequenceAcquisition();
>> }
>>
>> // RW ram store
>> storeRW = mm.data().createRewritableRAMDatastore();
>> // Create a display to show images as they are acquired.
>> mm.displays().createDisplay(storeRW);
>>
>> // actual acqusition in a try block to catch errors
>> try{
>> // start acqusition and run until it is stopped by command
>> // the sequence acquistion with preset number of Images did cause
>> problems
>> mmc.startContinuousSequenceAcquisition(0);
>> // Set up a Coords.CoordsBuilder for applying coordinates to each image.
>> builder = mm.data().getCoordsBuilder().z(0).channel(0).stagePosition(0);
>>
>> while (mmc.getRemainingImageCount() > 0 ||
>> mmc.isSequenceRunning(mmc.getCameraDevice())) {
>> if (mmc.getRemainingImageCount() > 0) {
>> timeStart = System.currentTimeMillis();
>> tagged = mmc.popNextTaggedImage();
>> timeEnd = System.currentTimeMillis();
>> taggedTime = taggedTime + (timeEnd - timeStart);
>> frameNumber = tagged.tags.getInt("ImageNumber");
>> // stop sequence after nrFrames images
>> if (frameNumber >= nrFrames) {
>> timeStart = System.currentTimeMillis();
>> print("stop sequence");
>> mmc.stopSequenceAcquisition();
>> print("sequence stopped");
>> timeEnd = System.currentTimeMillis();
>> endSequenceTime = taggedTime + (timeEnd - timeStart);
>> }
>> // for visualisation
>> image = mm.data().convertTaggedImage(tagged,
>> builder.time(0).build(), null);
>> storeRW.putImage(image);
>> print("image Nr.: " + frameNumber);
>> loopCount++;
>> }
>> else {
>> java.lang.Thread.sleep(loopSleepTime);
>> }
>> }
>> }
>> catch (Exception exception) {
>> print(exception + " - something went wrong");
>> }
>> if (mmc.isSequenceRunning()) {
>> mmc.stopSequenceAcquisition();
>> }
>> storeRW.close();
>> print ("acqusition done");
>> print ("sum tagged time: " + taggedTime);
>> print ("end sequence time: " + endSequenceTime);
>>
>>
>> Thanks for helping me.
>>
>> kind regards Lukas
>>
>>
>> On 2020-03-12 10:07, Pedro Almada wrote:
>>> Hi Lukas,
>>>
>>> Just as a sanity check, have you set the circular memory buffer to
>>> an appropriate size?
>>>
https://micro-manager.org/wiki/Micro-Manager_Configuration_Guide#Memory_Settings
>>> <
https://urldefense.proofpoint.com/v2/url?u=https-3A__micro-2Dmanager.org_wiki_Micro-2DManager-5FConfiguration-5FGuide-23Memory-5FSettings&d=DwMDaQ&c=iORugZls2LlYyCAZRB3XLg&r=UwP8SWqih8VHO1LwZpgcx83I4o21yLj6V6QD-25Dt4I&m=Azgfttsqypz0DvZFBuWwdGg8HC4NaQyiaa1fqvYEv0A&s=PG8_MP-bp5ljDiggl6RgaGpdZCvv5zr-LEynV0WaowQ&e=>
>>>
>>>
>>> Kind regards,
>>> Pedro Almada
>>>
>>> On Wed, 11 Mar 2020 at 18:04, Lukas Hille <
[hidden email]
>>> <mailto:
[hidden email]>> wrote:
>>>
>>> Hi,
>>>
>>> I need help with two Andor cameras.
>>>
>>> Two Andor cameras are used in dual view (Multi Camera adapter with
>>> Andor: iXon+ and Andor: iXon3).
>>> Acquisition is done by a burstAcquisition macro (equal to the
>>> script),
>>> but the described behavior applies also to MDA.
>>> With Frame transfer enabled and at higher Acquisition speeds
>>> (binning 2,
>>> 0.9us vertical clock, 20ms exposure, full ROI) i get an buffer
>>> overflow.
>>>
>>> It appears that the device adapter before 14.April2019 "old"
>>> returns
>>> very quick from mmc.popNextTaggedImage() and therefore produces no
>>> buffer overflow, while the adapter after 14.April2019 "new" takes
>>> a very
>>> long time to return from this function.
>>> This also occurs with only one Camera in use, but with two
>>> cameras the
>>> return time is to long to handle the data flow.
>>>
>>> A second issue is, that with Multi Camera adapter, the camera
>>> referring
>>> to channel 0 (independent which one) stops acquiring after some
>>> time
>>> (~60.000 images in Trigger: software).
>>> This applies to the "old" and the "new" Andor device adapter.
>>>
>>> With Multi Camera adapter and external trigger (one source for both
>>> cameras, Trigger set to External on both cameras), the camera
>>> referring
>>> to channel 0 stops acquiring within 0 to some thousand images)
>>>
>>> I don't get any debugging error message with the new adapter.
>>> With the old adapter i get for example: "GetNumberNewImages error:
>>> first: 3473 last: 3473" for the camera on channel 0 before it
>>> stops working.
>>>
>>> The goal would be to acquire about 200,000 time points with
>>> external
>>> trigger at 20ms exposure time.
>>>
>>> I tried to understand the differences in the code from commits on
>>> 14.April, but I didn't figure out why this makes a difference for
>>> the
>>> popNextTaggedImage() function.
>>> Also: the error message "GetNumberNewImages error:" doesn't make
>>> any
>>> sense to me, referring to the situation that no new images are
>>> acquired.
>>>
>>> I would be very happy to get assistance on this one.
>>>
>>> Software:
>>> AndorDriverPack2 - 2.103.30031.0
>>> Micro-Manager 2.0.0-gamma1 20200222
>>> mmgr_dal_Andor.dll from 1.04.2019, 16.04.2019 and 22.02.2019
>>> (tried many
>>> many more)
>>>
>>> best
>>> Lukas
>>>
>>> -- ____________________________________________
>>> Lukas Hille, M.Sc.
>>> microscopy engineer
>>>
[hidden email] <mailto:
[hidden email]>
>>> M +43 660 349 169 3
>>>
>>> Department of Neurobiology, University of Vienna
>>> Campus-Vienna-Biocenter 1
>>> 1030 Vienna
>>> AUSTRIA
>>>
>>>
https://neuro.univie.ac.at/>>> <
https://urldefense.proofpoint.com/v2/url?u=https-3A__neuro.univie.ac.at_&d=DwMDaQ&c=iORugZls2LlYyCAZRB3XLg&r=UwP8SWqih8VHO1LwZpgcx83I4o21yLj6V6QD-25Dt4I&m=Azgfttsqypz0DvZFBuWwdGg8HC4NaQyiaa1fqvYEv0A&s=vOEXQZsKGgi3nnbjzvgQn42ESKwizdX8-w-cF9pJs3k&e=>
>>>
https://www.imp.ac.at/groups/manuel-zimmer/>>> <
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>>> Part of Vienna BioCenter
>>> www.viennabiocenter.org
>>> <
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.viennabiocenter.org&d=DwMDaQ&c=iORugZls2LlYyCAZRB3XLg&r=UwP8SWqih8VHO1LwZpgcx83I4o21yLj6V6QD-25Dt4I&m=Azgfttsqypz0DvZFBuWwdGg8HC4NaQyiaa1fqvYEv0A&s=JQvGS7Y-Uc6IqcKD3lPntn5yVlZO0i7rpltQBfj9trE&e=>
>>> ____________________________________________
>>>
>>>
>>>
>>> _______________________________________________
>>> micro-manager-general mailing list
>>>
[hidden email]
>>> <mailto:
[hidden email]>
>>>
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>>> micro-manager-general mailing list
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https://lists.sourceforge.net/lists/listinfo/micro-manager-general>> --
>> ____________________________________________
>> Lukas Hille, M.Sc.
>> microscopy engineer
>>
[hidden email]
>> M +43 660 349 169 3
>>
>> Department of Neurobiology, University of Vienna
>> Campus-Vienna-Biocenter 1
>> 1030 Vienna
>> AUSTRIA
>>
>>
https://neuro.univie.ac.at/>>
https://www.imp.ac.at/groups/manuel-zimmer/>> Part of Vienna BioCenter
>> www.viennabiocenter.org
>> ____________________________________________
>>
>>
>> _______________________________________________
>> micro-manager-general mailing list
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