Hi Dan,
> I would like to use something similar to flat-field correction on some
> polarization contrast imaging. Currently, flat-field correction
> calculates on-the-fly:
>
> Resulting image = (acquired image – background) / (flatfield image –
> background)
>
> I want:
>
> Resulting image = acquired image – background
>
At least in 2.0-gamma, simply leave out the flatfield image, and you
will get on the fly background subtraction. It can even be carried out
on the GPU (first GPU accelerated code in MM, more to follow). I don't
know/remember behavior in older MM versions, but you can easily test.
Switching to gamma should be a good thing in any case.
Best,
Nico
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