Hi Brian,
> I am working with a Labview wrapper of Micromanager. I need to acquire images as fast as possible from a QuantEM. As I understand it, this should be done by streaming the images into micromanager and then reading from the circular buffer, but I can only figure out how to do this by acquiring X images and then reading them out after they have been acquired. This is not possible for my application as I need to acquire 500 images and review them as the images are taken. Is there a way to set the camera into streaming mode and then read out the images as they are created?
I wrote the Beanshell script copied below as an example. Most of it deals with getting the images into the Micro-Manager viewer and you will not care about it. The important part is:
mmc.startSequenceAcquisition(nrFrames, 0, false);
while (mmc.getRemainingImageCount() > 0 || mmc.isSequenceRunning(mmc.getCameraDevice())) {
if (mmc.getRemainingImageCount() > 0) {
img = mmc.popNextImage();
// do whatever you want with the image
}
}
Note that mmc.startSequenceacquistion() is non-blocking, so you should have no trouble analyzing the images as they are acquired.
Hope this helps!
Nico
/*
* Script to show how to use Sequence acquisition
*/
// file locations
acqName = "test-Burst";
rootDirName = "C:/acquisitionData";
// Number of timepoints
nrFrames = 50;
// clear all previous acquisitions
gui.closeAllAcquisitions();
gui.clearMessageWindow();
gui.openAcquisition(acqName, rootDirName, nrFrames, 1, 1);
long width = mmc.getImageWidth();
long height = mmc.getImageHeight();
long depth = mmc.getBytesPerPixel();
gui.initializeAcquisition(acqName, (int) width, (int) height, (int) depth);
exposure = mmc.getExposure();
binning = mmc.getProperty(mmc.getCameraDevice(), "Binning");
mmc.startSequenceAcquisition(nrFrames, 0, false);
frame = 0;
now = System.currentTimeMillis();
while (mmc.getRemainingImageCount() > 0 || mmc.isSequenceRunning(mmc.getCameraDevice())) {
if (mmc.getRemainingImageCount() > 0) {
img = mmc.popNextImage();
gui.addImage(acqName, img, frame, 0,0);
frame++;
}
}
itTook = System.currentTimeMillis() - now;
mmc.stopSequenceAcquisition();
gui.message("Acquisition took: " + itTook + " milliseconds");
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