Flat field via on-the-fly not working in 1.4?

classic Classic list List threaded Threaded
1 message Options
Reply | Threaded
Open this post in threaded view

Flat field via on-the-fly not working in 1.4?

Chris Gell

Dear all,


I would like to use an on-the-fly processor to perform a flat field (shading) correction with bright field (and also fluorescence) in v1.4.


Unless I am doing something silly, the  pipeline is ignored in 1.4.23 20170207; this can be tested using MMConfig_demo.cfg, using a snap of the periodic simulated-image as the ff image. (Other pipelines, such as image flipper work.)


It’s quite possible I am doing something wrong, however the same workflow in the 2.0 beta does work correctly.


Could someone try this out for me in 1.4.23….










Dr Chris Gell

School of Life Sciences Imaging and Microscopy (SLIM) Facility,

Advanced Microsocpy Unit (AMU)

Room E100

University of Nottingham

The Medical School





Office:   +44 (0)115 8230297

Mobile: 0777 848 4019

[hidden email]


Follow us on Twitter @UoN_AMU


This message and any attachment are intended solely for the addressee
and may contain confidential information. If you have received this
message in error, please send it back to me, and immediately delete it. 

Please do not use, copy or disclose the information contained in this
message or in any attachment.  Any views or opinions expressed by the
author of this email do not necessarily reflect the views of the
University of Nottingham.

This message has been checked for viruses but the contents of an
attachment may still contain software viruses which could damage your
computer system, you are advised to perform your own checks. Email
communications with the University of Nottingham may be monitored as
permitted by UK legislation.

Check out the vibrant tech community on one of the world's most
engaging tech sites, SlashDot.org! http://sdm.link/slashdot
micro-manager-general mailing list
[hidden email]