Live mode & shutter control issue

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Live mode & shutter control issue

PEARSON Matthew
Hi there,

I've downloaded the latest nightly build of Micro-Manager 2 (180118) and have a problem when updating the exposure time in the GUI.  This is what i'm seeing:
-Enter an exposure time in the GUI
-Press Refresh button
-Press Live
-Live window opens but shutter doesn't (Autoshutter is enabled)
-Stop Live
-Press Live again shutter opens this time and all subsequent times until exposure is changed again

I'm not sure if this is a universal issue that everyone using this build would encounter or whether its specific to our hardware.  The shutter controller is a Ludl Mac 2000.

Thanks for the help,

Matt


--
Matt Pearson
Microscopy Facility
MRC Human Genetics Unit
Institute of Genetics and Molecular Medicine (IGMM)
University of Edinburgh
Crewe Road
EH4 2XU





The University of Edinburgh is a charitable body, registered in
Scotland, with registration number SC005336.

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Re: Live mode & shutter control issue

Amit Cherian
We hav this issue as well (MM version 1.4.23.20170521). We tried with a
couple of later versions MM but the issue seemed to be persistent. We
assumed it was a hardware specific issue as this happened only with the
Agilent laser combiner being part of the MM configuration. The work-around
we found was to have the camera trigger the laser instead of having the
laser triggered by MM.

But now with your post it seems like it may not be a hardware issue after
all.

Amit Cherian
Junior Research Fellow,
Satyajit Mayor Lab




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Re: Live mode & shutter control issue

PEARSON Matthew
Thanks amit.  Triggering isn't really a line we want to go down for this application but glad that worked for you.  This seems like quite a fundamental issue but as you say maybe hardware dependent.  I have ruled out the camera or its driver being at fault as the new exposure times are taken up and updated but i guess i can't rule out that this could be involved perhaps.. That is a Photometrics Coolsnap HQ2.

Thanks,

Matt

 
--
Matt Pearson
Microscopy Facility
MRC Human Genetics Unit
Institute of Genetics and Molecular Medicine (IGMM)
University of Edinburgh
Crewe Road
EH4 2XU




On 22 Jan 2018, at 11:53, Amit Cherian <[hidden email]> wrote:

We hav this issue as well (MM version 1.4.23.20170521). We tried with a
couple of later versions MM but the issue seemed to be persistent. We
assumed it was a hardware specific issue as this happened only with the
Agilent laser combiner being part of the MM configuration. The work-around
we found was to have the camera trigger the laser instead of having the
laser triggered by MM.

But now with your post it seems like it may not be a hardware issue after
all.

Amit Cherian
Junior Research Fellow,
Satyajit Mayor Lab




--
Sent from: http://micro-manager.3463995.n2.nabble.com/

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Multi-dimensional acquisition : lower objective between positions

Vaughan, Alexander
In reply to this post by Amit Cherian
Hi,

Is it possible to lower the objective a few mm between imaging positions?

We use a specialized stage/heat-plate, and I would like to avoid banging my objective on the bottom of the heat-plate when switching positions.

Is this currently possible?  If not, could you give me a sense of the best way to change code to make this possible?

Many thanks,
Alex


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Re: Multi-dimensional acquisition : lower objective between positions

Ti_Sapph
Alex,

This would be straightforward to do in a Beanshell script.  You would set up a script to do the usual multi-position acquisition and include a drop of x mm before the next position iteration. 

Thanks,
Rusty

On Mon, Jan 22, 2018 at 8:00 AM, Vaughan, Alexander <[hidden email]> wrote:
Hi,

Is it possible to lower the objective a few mm between imaging positions?

We use a specialized stage/heat-plate, and I would like to avoid banging my objective on the bottom of the heat-plate when switching positions.

Is this currently possible?  If not, could you give me a sense of the best way to change code to make this possible?

Many thanks,
Alex


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