Monitoring changes in fluorescence intensity (online)
Dear Micro-Manager users,
I'm a new user, and I used to use HCImage to monitor calcium trasients in neurons.
As not everything goes the way we expect, I had to change and use another set-up for calcium imaging, of which there is only Micro-Manager available. I've been reading many posts in this forum, and I found some plugins, none of which I'd be able to monitor changes in fluorescence intensity over time online, as I'm going to stimulate neuron cultures through perfusion with high potassium concentration solution. Do you have a plugin that can do that, or something that I could work on? Could you share it with me?