Multi-D bug??

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Multi-D bug??

Jonathan Compton
Hello,

I am trying to take a z-stack at multiple locations using the xy list. I select relative z and acquire, and it acts as if I chose absolute z by using the predefined range as the actual Z positions. I then un-check the use xy list button at a location and then it works correctly using the relative z range. Is this a bug or what?

--
Jon


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Re: Multi-D bug??

Arthur D. Edelstein
Hi Jon,

That does sound like a bug, but I can't seem to reproduce it. Can you
send me an MMAcquisition.xml file and your CoreLog.txt file? You can
create the xml file by pressing the Save... button in the
multi-dimensional acquisition dialog.

Best regards,
Arthur



On Wed, Dec 9, 2009 at 11:17 PM, Jonathan Compton
<[hidden email]> wrote:

> Hello,
>
> I am trying to take a z-stack at multiple locations using the xy list. I
> select relative z and acquire, and it acts as if I chose absolute z by using
> the predefined range as the actual Z positions. I then un-check the use xy
> list button at a location and then it works correctly using the relative z
> range. Is this a bug or what?
>
> --
> Jon
>
>
> ------------------------------------------------------------------------------
> Return on Information:
> Google Enterprise Search pays you back
> Get the facts.
> http://p.sf.net/sfu/google-dev2dev
>
> _______________________________________________
> micro-manager-general mailing list
> [hidden email]
> https://lists.sourceforge.net/lists/listinfo/micro-manager-general
>
>

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Re: Multi-D bug??

Jonathan Compton
In reply to this post by Jonathan Compton
Here is the information you requested. I wasn't sure if I send it to the forum or you as I didnt know what attaching would do.
 
 
Here is what I do.
 
I have 5 positions saved on the XY list, and I want to do a relative z-stack at say +1 to +4 um.
 
I start the aquisition and the first position works fine, then the following positions have the error. They go to the preset Z for the initial frame it seems then the following frames jump to either the Z of the first position or the actual z-stack values, ie 1-4um or whatever the preset is for the first position plus the stack numbers.
 
I hope this helps.

On Wed, Dec 9, 2009 at 11:17 PM, Jonathan Compton <[hidden email]> wrote:
Hello,

I am trying to take a z-stack at multiple locations using the xy list. I select relative z and acquire, and it acts as if I chose absolute z by using the predefined range as the actual Z positions. I then un-check the use xy list button at a location and then it works correctly using the relative z range. Is this a bug or what?

--
Jon




--
Jonathan Compton
Graduate Student Chemical Engineering
UC Irvine


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CoreLog.txt (28K) Download Attachment
MMAcquistion.xml (3K) Download Attachment
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Re: Multi-D bug??

Arthur D. Edelstein
Hi Jon,

Thanks for the reminder and sorry about the delay. I have found the
bug you reported and I believe I have fixed it. A corrected version of
Micro-Manager for Windows should be available in the latest nightly
build tomorrow morning (let me know if you need the Mac version):

http://valelab.ucsf.edu/~MM/nightlyBuilds/

Thanks again for reporting this. Please let me know if you have
further problems.

Best regards,
Arthur



On Mon, Jan 4, 2010 at 2:37 PM, Jonathan Compton
<[hidden email]> wrote:

> Here is the information you requested. I wasn't sure if I send it to the
> forum or you as I didnt know what attaching would do.
>
>
> Here is what I do.
>
> I have 5 positions saved on the XY list, and I want to do a relative z-stack
> at say +1 to +4 um.
>
> I start the aquisition and the first position works fine, then the following
> positions have the error. They go to the preset Z for the initial frame it
> seems then the following frames jump to either the Z of the first position
> or the actual z-stack values, ie 1-4um or whatever the preset is for the
> first position plus the stack numbers.
>
> I hope this helps.
>
> On Wed, Dec 9, 2009 at 11:17 PM, Jonathan Compton
> <[hidden email]> wrote:
>>
>> Hello,
>>
>> I am trying to take a z-stack at multiple locations using the xy list. I
>> select relative z and acquire, and it acts as if I chose absolute z by using
>> the predefined range as the actual Z positions. I then un-check the use xy
>> list button at a location and then it works correctly using the relative z
>> range. Is this a bug or what?
>>
>> --
>> Jon
>>
>
>
>
> --
> Jonathan Compton
> Graduate Student Chemical Engineering
> UC Irvine
>
>
> ------------------------------------------------------------------------------
> This SF.Net email is sponsored by the Verizon Developer Community
> Take advantage of Verizon's best-in-class app development support
> A streamlined, 14 day to market process makes app distribution fast and easy
> Join now and get one step closer to millions of Verizon customers
> http://p.sf.net/sfu/verizon-dev2dev
> _______________________________________________
> micro-manager-general mailing list
> [hidden email]
> https://lists.sourceforge.net/lists/listinfo/micro-manager-general
>
>

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