I am just wondering about
the coordinates for the software and the actual microscope. For the micro
manager, our origin starts at the top left corner while the Olympus (microscope)
origin starts at the middle of the plate. It gets really hard to take images
since when the software thinks its on the first well, the actual microscope
plate has reached the bottom left corner.
Additionally, for the
software or Micro Manager on the HSC site generator, the x is positive when it
moves right and y is positive when it moves down. For the actual plate or stage
of the microscope, x is positive when it moves left and y is positive when it
We have thought about
looking at the codes and maybe fix it from there but we don't know how to do
Any suggestions on how to
fix this will be great. This is my first time using this software so I apologize
for the lack of technical language.