exposure > 1000 ms?

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exposure > 1000 ms?

Matthew Fleming
Hi,

I've just started using micromanager with a Tucsen TCC-1.4HICE cooled monochrome camera. It works, except for two problems: 1. I am unable to increase the exposure beyond 1000 ms 2. Changes to the exposure, etc, are not immediately reflected in the live image view. In order to see them, I have to stop and restart the live image display.

Would very much appreciate any suggestions as to how to resolve these issues.

Thank you,

Matthew Fleming, MD
Fleming Dermatopathology, LLC


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Re: exposure > 1000 ms?

Thorn, Kurt
My guess is that the 1000 ms limit is either in the camera driver or in the camera itself as we routinely use exposures over 1 sec with other cameras.  It might be worth contacting the camera manufacturer directly about this.

Kurt

On 10/26/2011 8:12 AM, Matthew Fleming wrote:
Hi,

I've just started using micromanager with a Tucsen TCC-1.4HICE cooled monochrome camera. It works, except for two problems: 1. I am unable to increase the exposure beyond 1000 ms 2. Changes to the exposure, etc, are not immediately reflected in the live image view. In order to see them, I have to stop and restart the live image display.

Would very much appreciate any suggestions as to how to resolve these issues.

Thank you,

Matthew Fleming, MD
Fleming Dermatopathology, LLC



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Re: exposure > 1000 ms?

Nico Stuurman-4
In reply to this post by Matthew Fleming
On Oct 26, 2011, at 8:12 AM, Matthew Fleming wrote:

> I've just started using micromanager with a Tucsen TCC-1.4HICE cooled monochrome camera. It works, except for two problems: 1. I am unable to increase the exposure beyond 1000 ms 2.

What adapter are you using to interface with that camera?

> Changes to the exposure, etc, are not immediately reflected in the live image view. In order to see them, I have to stop and restart the live image display.

When I change exposure and press the Enter key, changes are immediately reflected in the live stream.

Best,

Nico


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warning about plus sign in image name, at least in Windows

Cammer, Michael
In reply to this post by Matthew Fleming

I got a late night text message from one of my colleagues that her files were not saving when using the Multi Dimension Acquire window.  When I checked this morning, I found that she had a file name something like, "PKC +GFP" and that the files were being saved, but over and over in the same directory without incrementing the directory number so she only had the most recent acquisition on disk.  When I replaced the "+" with "plus", it worked fine.

 

________________________________________________________

Michael Cammer, Assistant Research Scientist

Skirball Institute of Biomolecular Medicine

Lab: (212) 263-3208  Cell: (914) 309-3270

 

 




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Re: warning about plus sign in image name, at least in Windows

Gordon Scott-2

FWIW, I always try to avoid anything in filenames that could be a special character on some machine. That means I tend to stick to alphanumeric, dot and underscore. I do use minus, which is usually harmless within a filename, provided it’s not the first character or two. Outside of that, you’re always at risk of some application or OS messing up the filename parsing.

 

Gordon.

 

From: Cammer, Michael [mailto:[hidden email]]
Sent: 27 October 2011 21:04
To: [hidden email]
Subject: [micro-manager-general] warning about plus sign in image name, at least in Windows

 

I got a late night text message from one of my colleagues that her files were not saving when using the Multi Dimension Acquire window.  When I checked this morning, I found that she had a file name something like, "PKC +GFP" and that the files were being saved, but over and over in the same directory without incrementing the directory number so she only had the most recent acquisition on disk.  When I replaced the "+" with "plus", it worked fine.

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Re: warning about plus sign in image name, at least in Windows

Johannes Schindelin
Hi,

On Fri, 28 Oct 2011, Gordon Scott wrote:

> From: Cammer, Michael [mailto:[hidden email]]
>
> > I got a late night text message from one of my colleagues that her
> > files were not saving when using the Multi Dimension Acquire window.  
> > When I checked this morning, I found that she had a file name
> > something like, "PKC +GFP" and that the files were being saved, but
> > over and over in the same directory without incrementing the directory
> > number so she only had the most recent acquisition on disk.  When I
> > replaced the "+" with "plus", it worked fine.
>
> FWIW, I always try to avoid anything in filenames that could be a
> special character on some machine. That means I tend to stick to
> alphanumeric, dot and underscore. I do use minus, which is usually
> harmless within a filename, provided it's not the first character or
> two. Outside of that, you're always at risk of some application or OS
> messing up the filename parsing.
The plus sign is reserved on Windows for file concatenation, that is why
you cannot use it. For similar reasons, you should stay away from slashes,
backslashes, colons, semicolons, newlines (you would be surprised what
crap users want to put into a filename) and basically any esoteric
character. Unicode characters such as ♥, ±, ℵ, ☂ ,☎ , or ♬  are also
a bad idea.

In general, I recommend to users to treat filenames like children when
giving them names. Metadata should not be part of the filename. If at all,
use subdirectories' names for things like dates or treatments, but in the
end you will always realize that the best way to manage metadata is by
putting the files into a special-purpose database such as OMERO.

Ciao,
Johannes

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